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In vitro anti-inflammatory and anti-microbial activities of ethanol extract from Cnidoscolus chayamansa (Mc Vaugh) leaves
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| Author:
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KANITTADA THONGKAO, NARIN KAKATUM, PIMPORN THONGMUANG, YUTTANA SUDJAROEN
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| Abstract:
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Cnidoscolus chayamansa (Mc Vaugh) is belonging to Euphorbiaceae family and local name is “Mexican spinach” or “chaya”. There is a leafy green vegetable in dry tropical areas, which used as high nutritive food and ethnomedicinal applying. C. chayamansa tree is trend to more cultivate in Thailand as high nutritive food and ethnomedical aspect. Aims of this study were to evaluate In vitro anti-inflammation; and to evaluate anti-microbial activity of ethanol extract from C. chayamansa leaves. Fresh leaves were cleaned, air dried, and extracted with 95% ethanol by maceration. Total phenolic content (TPC) measurement of C. chayamansa leaves extract was determined. In vitro inflammatory activity of extracts was evaluated and compared with diclofenac diethylammonium. Anti-microbial activity of C. chayamansa leaves extract was evaluated against pathogenic bacteria, Streptococcus mutans, Staphylococcus aureus and Propionibacterium acnes; and pathogenic yeasts, Candida albicans and Malassezia furfur by disc diffusion method. TPC contained in ethanol extract from C. chayamansa leaves was 14.32±0.59 mg GAE/g. Anti-inflammation activities of C. chayamansa leaves extract was preferable high (IC50 = 3.09±0.06 mg/ml) compared with diclofenac diethylammonium. C. chayamansa leaves extract was exhibited antimicrobial activity against tested Gram ?s positive bacteria including S. mutans, S. aureus, and P. acnes; and activity was slightly active. However, C. chayamansa leaves extract was not inhibited C. albicans and M. furfur. Combining of anti-inflammation and anti-bacterial activities, especially skin bacterial pathogens from C. chayamansa leaves had supporting data on topical medicinal use for relieving skin inflammation and infection.
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| Keyword:
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anti-bacterial activity, anti-inflammation, anti-microbial, chaya leaf, Cnidoscolus chayamansa (Mc Vaugh), Mexican spinach
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.664
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| Download:
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