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Interaction of Blastema Cells In 3d Pancreatic Scaffold In Vitro
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| Author:
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FAHIMEH JAMSHIDI, SAIIDEH SAMAREH-MOOSAVI, KAMELIA KOHANNEZHAD
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| Abstract:
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Pancreas bioengineered requires a scaffold that approximates the biochemical, spatial and vascular relationships of the native extracellular matrix (ECM). Proteoglycans (PGs) in the ECM binds to several signaling molecules and regulates ligand- receptor interaction, playing an essential role in embryonic development. Pancreatic bovine tissue (5 mm×5 mm×5 mm) was decellularized using physical methods (slow freezing and snap freeze– thaw) and chemical agents (sodium dodecyl sulfate (SDS) and Triton ?-100) followed by washing and sterilization procedures. The scaffolds were assembled within the blastema ring that obtained from pinna rabbit punch in order to investigate their interactions in three– dimensional conditions in vitro. The samples were studied histochemicaly after 7, 14, 21 and 27 days in culture. Interaction between blastema and scaffolds indicated that the best time for studding the interactions was shown to be 14 and 21 days after the co-culture. Designed scaffold with these properties may have useful applications in tissue engineering and regenerative medicine.
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| Keyword:
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Tissue engineering, Decellularization, Bovine pancreas, Blastema
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.750
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| Download:
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Request For Article
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