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Nephroprotective Activity of Annona Squamosa Extract on Amphotericin B-Induced Nephrotoxicity through InVitro and In-Vivo Models
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| Author:
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NEELIMA S, DWARAKANADHA REDDY P, CHANDRA SEKHAR KOTHAPALLI BANNOTH
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| Abstract:
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Amphotericin B (ATB) is one of the extensively used antifungal agent against many fungal infections. The
excess intake of ATB exhibits the nephrotoxicity due to oxidative stress. Annona squamosa (AS), which showed
many therapeutic strengths was chosen for the present study to investigate on its nephroprotective
effect.Major objective of this study was to estimate the nephroprotective effect of ethanolic extract of Annona
squamosa leaves (EAS) towards ATB-induced nephrotoxicity with an aid of in vitro Human Embryonic Kidney
(HEK)-293 cells and in vivo on Wistar rats. In vitro cytoprotective effect was assessed by MTT assay using HEK-
293 cells. Male Wistar rats (N=24) were alienated into four groups with six animals each. Group I given
normal saline known as normal control. Group II was disease control, administered 15mg/kg of ATB, i.p. daily
once for 5 days; Group III & IV animals were pre-treated with EAS of dose 200 & 400 mg/kg p.o. for ten days
and from 6th day of experiment, animals began to receive ATB at 15mg/kg i.p., single dose for 5 days. HEK-293
cells incubated with ATB exhibited an increased cell death and were decreased on treatment with EAS. A
quantitative estimation of ALT, AST and ALP, creatinine and blood urea nitrogen (BUN) were used to asses in
vivo nephroprotective activity of EAS. The reduction (P<0.001) of serum biomarkers and elevation of
glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD) levels (P<0.001) in kidney tissue
homogenate in treated groups when compared to the ATB treated alone was observed. Results were also
supplemented by histopathological investigations. The work concludes potential of EAS against ATB-induced
nephrotoxicity via in vitro and in vivo trials
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| Keyword:
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Nephroprotective, Amphotericin B, Annona squamosa, MTT assay, Oxidative stress.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.454
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| Download:
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