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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Nephroprotective Activity of Annona Squamosa Extract on Amphotericin B-Induced Nephrotoxicity through InVitro and In-Vivo Models

Author: NEELIMA S, DWARAKANADHA REDDY P, CHANDRA SEKHAR KOTHAPALLI BANNOTH
Abstract: Amphotericin B (ATB) is one of the extensively used antifungal agent against many fungal infections. The excess intake of ATB exhibits the nephrotoxicity due to oxidative stress. Annona squamosa (AS), which showed many therapeutic strengths was chosen for the present study to investigate on its nephroprotective effect.Major objective of this study was to estimate the nephroprotective effect of ethanolic extract of Annona squamosa leaves (EAS) towards ATB-induced nephrotoxicity with an aid of in vitro Human Embryonic Kidney (HEK)-293 cells and in vivo on Wistar rats. In vitro cytoprotective effect was assessed by MTT assay using HEK- 293 cells. Male Wistar rats (N=24) were alienated into four groups with six animals each. Group I given normal saline known as normal control. Group II was disease control, administered 15mg/kg of ATB, i.p. daily once for 5 days; Group III & IV animals were pre-treated with EAS of dose 200 & 400 mg/kg p.o. for ten days and from 6th day of experiment, animals began to receive ATB at 15mg/kg i.p., single dose for 5 days. HEK-293 cells incubated with ATB exhibited an increased cell death and were decreased on treatment with EAS. A quantitative estimation of ALT, AST and ALP, creatinine and blood urea nitrogen (BUN) were used to asses in vivo nephroprotective activity of EAS. The reduction (P<0.001) of serum biomarkers and elevation of glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD) levels (P<0.001) in kidney tissue homogenate in treated groups when compared to the ATB treated alone was observed. Results were also supplemented by histopathological investigations. The work concludes potential of EAS against ATB-induced nephrotoxicity via in vitro and in vivo trials
Keyword: Nephroprotective, Amphotericin B, Annona squamosa, MTT assay, Oxidative stress.
DOI: https://doi.org/10.31838/ijpr/2021.13.02.454
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