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The Effect of Diet Variation on Cadherin Expression Using CD 326 on Gastric Mucosa in Male Mice
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| Author:
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HAIDER A JAFFAR, HIBA M RASOOL
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| Abstract:
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This study aimed to correlate the effect of diet variation on the tight junctions (E-cadherin protein) that connect epithelial cells to each other) of gastric mucosa. A fifty (50) adult male mice (albino mice) were applied in this study, aged about 10-12 weeks, weighing between 20-30 g, collected from National Center for Drug Control and Research. They were underwent diet variety, whether group A: was fed on 0.5% protein in diet, group B was fed on 15% protein in diet, group C was fed on full protein diet, group D was fed on intermittent fasting program (fasting for 18 hours every 2 days and then fed on standard pellet) and control group was fed on standard pellet (30%protein). The experiment lasted for 30 days. These animals were euthanized by inhalation of chloroform in a closed glass jar then fixative solution 10% neutral buffered formalin was injected intra vascular through left ventricle into aorta through median thoracotomy. After that mice were dissected by median abdominal incision ventrally so that to harvest the stomach. The specimens of body region of stomach was transferred to 10% neutral buffered formalin (NBF) for 48 hours in order to obtain paraffin section for routine histological (H&E) and immunohistochemical (E-cadherin protein) staining. The thickness of the mucosa and the diameters of the gastric gland were measured by image j software program.
It was found that group C (fed on full protein diet) had the thickest mucosa (311.6±97.19 micron) and the widest gland diameter (22.46±3.2 micron) while group A (that fed on zero% protein in diet) revealed the lowest values (205±72.6)( 8.66±2.4 micron).
The E-cadherin protein expression of gastric mucosa of group C (fed on full protein diet) had the highest cadherin expression (0.8±0.1 pixels per micron) in comparison to the lowest expression (0.4±0.1 pixels per micron) of group A (that fed on zero% protein in diet). However, group D (fed on intermittent fasting program) had a slightly higher cadherin expression (0.6±0.3) than the control and this can be a protective and adaptation measure according to the change in diet habits (as stress condition). In conclusion, consuming a high protein diet can cause a decrease in the healthy turnover process of the gastric mucosa, on the other hand having a low protein diet can lead to atrophied (thin) mucosa.
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| Keyword:
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E-cadherin protein, tight junctions, CD 326, diet variation.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.724
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| Download:
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