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Isolation, Characterization and Identification of Phosphate Solubilizing Bacteria (PSB) In Natural Farming Paddy Soil in Salassae Village, Bulukumba Regence, South Sulawesi Province, Indonesia
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| Author:
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HADIJA , T.KUSWINANTI, M.JAYADI, SH LAREKENG, M.NADIR
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| Abstract:
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One of the most needed macronutrient elements is Phosphate (P). Still, most low availability in the soil, because most of the phosphate fertilizer added, does not leach into the ground making it unavailable to plants. Phosphate Solubilizing Bacteria (PSB) is one of the soil microorganisms that can improve Phosphate's soil's supply. This study aims to isolate, characterize, and identify phosphate solubilizing bacteria in paddy soils using the natural farming system to increase phosphate availability in the ground—this study using the approach method morphology, biochemistry and Identification molecular to characterize and identify PSB isolates. Results The isolation of bacteria from the research location was successful twenty-three bacterial isolates. Furthermore, the macroscopic characterization results vary considerably from the shape of the colony from irregular to rooted. Test Pospat's dissolving ability obtained at six the bacterial isolate with the best dissolving ability was found in LK. E 1 isolates that 6.71 ppm. Identification biochemistry to test the sensitivity using VITEK®. 02 that the selected isolates also can react with various enzymes such as Phosphatase, Glucose, Inulin, D-Mannitol, Esculin hydrolysis and Tetrazolium Red. Based on the sequencing results, 16rRNA LK.E.1 isolates identified as Bacillus Subtilis with 99% similarity.
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| Keyword:
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Phosphate solubilizing bacteria, Bacillus Subtillis, Organic Paddy fields
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.297
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| Download:
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