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Formulation and Characterization of Tyrosine Kinase Inhibitor Loaded NLCs: Application of 23 Full Factorial Design Approach
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| Author:
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DHAIRYSHEEL GHADGE, NAMDEO JADHAV
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| Abstract:
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Aim of this research was to create nanostructured lipid carriers (NLCs) of a poorly water-soluble drug erlotinib. NLCs filled with erlotinib were formulated with solvent diffusion method. Application of 23 full factorial design approach was carried out to study an effect of independent variables such as total lipid concentration, drug concentration and surfactant concentration on particle size, entrapment efficiency and cumulative drug release as dependent variables. Optimized batch F5 showed a particle size 193.5±5.2nm, entrapment efficiency 89.52±3.4% and drug release 84.27±3.7% at the end of 72 hours. The morphology of the NLCs was examined using a high resolution electron scanning microscope (HRSEM), an optimised batch showed a smooth surface spherical morphology. Drug loaded NLCs were tested further for polydispersity index, zeta potential, drug loading and invitro cytotoxicity study on Panc 1 cells. NLCs consisting of monosteol and labrafac are capable of capturing the poorly water-soluble drug erlotinib offering greater opportunities for oral delivery of erlotinib in treatment of pancreatic cancer.
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| Keyword:
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erlotinib, nanostrucutred lipid carriers, oral delivery, 23 full factorial design, Panc 1 cell activity, pancreatic cancer, tyrosine kinase.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.655
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| Download:
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