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Evaluation of the Use of Silver and Zinc Oxide Nanoparticles as Antibacterial Agents Against Fish Bacterial Pathogens
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| Author:
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NOHA M SABRY, SAHAR T M TOLBA, MEDHAT IBRAHIM, SAMAH M BASSEM, GAMILA E EL-TAWEEL, FAGR KH.ABDEL-GAWAD
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| Abstract:
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Nanotechnology recently provided useful insight into using a new antibacterial agent with synergistic effects of the bactericide mechanism toward some pathogenic microorganisms. For example, nanosilver and nano zinc oxide represent a class of essential substances that might be widely used in advanced research and health-related activities. In the present work, we prepared silver (AgNPs), zinc oxide (ZnONPs) nanoparticles, characterized their structures with “UV-Vis “Spectroscopy, X-ray diffraction (XRD) and High-Resolution Transmission Electron Microscopy (HR-TEM). We then evaluated their antibacterial activity by using different antibiotic discs against Aeromonas hydrophila and Pseudomonas aeruginosa (Gram-negative bacteria), and Staphylococcus aureus (Gram-positive bacteria) isolated from water and fish of Qarun and Manzala Lakes. Also, the minimal inhibitory concentration (MIC) was calculated by the macro dilution method”. The obtained results found that AgNPs inhibited the growth of both Gram-positive and Gram-negative bacterial isolates at a concentration of 170 µg/ml. However, ZnONPs inhibited the growth of at 500 µg/ml. Preliminary growth analysis results suggest that silver and zinc oxide nanoparticles have an inhibitory activity on Gram-positive and Gram-negative bacterial isolates. The present study exhibited synthesized (metal, metal oxide) nanoparticles. For potent antibacterial activity, the nanoparticles concentration was considered as a significant factor. Moreover, in low concentrations, Silver nanoparticles inhibited the growth of bacterial strains than zinc oxide nanoparticles. It can be concluded that the application of silver and zinc oxide nanoparticles as antibacterial agents is a promising tool for disease treatment in the field of fish aquaculture.
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| Keyword:
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Bacterial growth, metal nanoparticle, silver nanoparticle, antibiotics.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.435
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| Download:
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