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Effect of Glycyrrhiza Glabra as Motility Stimulant on Sperm Function for Patients with Varicocele and Diabetes Before and After In-Vitro Preparation for ICSI
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| Author:
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, ETHMAN HASHEM MOHAMMED, DR.AMOURA M.ABU-EL-NAGA, DR.SAAD SALIH ALDUJAILY
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| Abstract:
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Both diabetes mellitus and varicocele are the most common diseases that present in many infertile patients.
This study was conducted to overwhelm the negative response for the diabetes and varicocele semen by in
vitro activation techniques using a medium containing herbal motility stimulants (i.e.Glycyrrhiza glabra). The
medium used for in vitro activation was Hams F12 with 0.1% Glycyrrhiza glabra (Gg) and two techniques were
performed namely; wash and spin technique and density gradient technique. The patients were divided into
eight groups according to male infertility factors. The results showed that the mean of sperm concentration in
the eight groups by using two activation techniques with free-Gg medium and with 0.1% G. glabra medium,
was significantly (P<0.001) lower than that of before activation. Whereas, the percentage of sperm motility and
morphologically normal sperm(MNS) after activation by using density gradient technique with medium
containing 0.1% Gg was significantly (P<0.001) higher in all groups than that of before activation and after
activation by using wash and spin technique. Also, percentage of sperm DNA fragmentation, the number of
round cells and sperm agglutination percentage after activation by using density gradient technique with
medium containing 0.1% G. glabra were significantly (P<0.001) lower than that of before activation and after
activation by using wash and spin technique in all groups. According to the data of the present study, the best
results of certain sperm characters were noticed when using a medium containing 0.1% Gg with density
gradient technique for diabetic and varicocele men. This data can be utilized to enhance the output of diabetic
and varicocele patient through assisted reproductive technologies programs.
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| Keyword:
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Diabetes mellitus, Varicocele, Density gradient washing technique, Glycyrrhiza glabra.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.436
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| Download:
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