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Could Grapefruit Ameliorate the Oxidative Stress, Biochemical and Histopathological Alterations Induced by Prolonged Metronidazole Therapy in the Liver and Kidney of Male Rats?
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| Author:
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SAMER S.FOUAD, EATEMAD A.AWADALLA, MOHAMMED H.HASSAN, MAHA ABDELBAKY AHMED FAHMY, EMAAD ABDEL-KAHAAR, RANA A. ALI
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| Abstract:
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We aimed to compare antioxidant versus interaction properties of grapefruit (GP) in reversal of prolonged metronidazole (MTZ)- induced hepatic and renal alterations at both biochemical and histopathological levels. A total 60 male albino Wister rats were included and were divided randomly into 4 groups (n=15 each). The first group (control) received 1% DMSO, the second group (GP) received grapefruit juice, the third group (MTZ) received MTZ and the last group (MTZ+ GP) received MTZ combined with grapefruit juice for 60 days. Biochemical analysis of serum liver enzymes (ALP, AST and ALT) , urea and creatinine using colorimetric methods were performed. For both colorimetric assays of oxidative stress markers and for histopathological analyses, tissue samples including the liver and kidneys were used. Significantly higher serum liver enzymes in MTZ group and MTZ+GP groups compared to control and GP groups with higher levels in MTZ+GP, p?0.05.Significantly higher serum levels of urea and creatinine among MTZ+GP group compared to other groups. Significantly higher oxidative stress index of liver and kidney tissue homogenates in both MTZ group and MTZ+GP groups compared to control and GP groups with higher levels in MTZ+GP, with significantly lower values in GP group compared to other groups, p?0.05. Regressive histological changes in the liver and kidneys of MTZ have shown these effects and more significant in MTZ+GP group, with histological similarities between control and GP groups. Although GP has potent antioxidant effect but its interaction properties deteriorate MTZ- induced adverse liver and kidney changes at both biochemical and histological levels.
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| Keyword:
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metronidazole; grapefruit; Liver; Kidney; experimental animals; oxidative stress.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.417
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| Download:
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Request For Article
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