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Ameliorating effect of urokinase in bleomycin- induced pulmonary fibrosis in rats
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| Author:
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MAHDI M.THUWAINI, ALI ESMAIL AL-SNAFI, HANAA S.KHADEM
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| Abstract:
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Pulmonary fibrosis (PF) is a chronic lung disorder that may be produced by infections, toxic agents, immune-mediated, or might be idiopathic. It frequently progressive with marked interstitial lung damage, ultimately fibrosis, and loss of the lung elasticity ended by respiratory failure.The objective of the study is to investigate the protective effects of urokinase administration during bleomycin treatment.A total of 32 male adult albino rats were equally distributed into four groups and treated as follow, Group1: rats received 0.5 ml normal saline / rat/ day ip., to serve as control. Group2: rats received bleomycin 15mg/kg bw ip., three times weekly for 8wks. Group3: rats received urokinase (uPA)10mg/ kg bw as a single dose daily for 8wks. Group4: rats received bleomycin and urokinase simultaneously at doses, route and periods similar to groups 2 and 3 mentioned above.At the end of the experiment, rats were anaesthetized with chloroform and venous blood samples were collected for estimation of serum SOD, GSHpx and HYP. After determination of relative lung weight, lung tissue samples were taken for histological examination. The current study showed that the lungs relative weight (mg/100g body weight) was significantly increased (P<0.01) in BLM treated group in comparison with control group. Urokinase alone did not induced significant changes in the lung relative weight(P<0.31) in comparison with control. However, when it used with BLM, it significantly decreased (P<0.01) the lung relative weight in comparison to BLM group. On other hand, bleomycin significantly increased (P<0.001) seum HYP level, while, urokinase, in combination with BLM significantly ameliorated (P<0.03) HYPlevel. Histologically, the lungs of rat treated with bleomycin and urokinase showed reduced both, inflammatory cells infiltration and thickness of the interalveolar septa, and reduction of interalveolar deposition of collagen compared to bleomycin group. The current study revealed that the urokinase amelioratedthe pulmonary fibrosis induced by bleomycin in rats, and its use in combination with bleomycin significantly changed the biochemical parameters and reduced the histological lesions induced by bleomycin.
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| Keyword:
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Urokinase, bleomycin, pulmonary fibrosis, hydroxyprolin
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.339
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| Download:
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