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Bacterial Contaminants and Antimicrobial Susceptibility Pattern from Tiny Freshwater Shrimp (Macrobranchium Lanchesteri)
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| Author:
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KANITTADA THONGKAO, YUTTANA SUDJAROEN
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| Abstract:
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Tiny freshwater shrimp (Macrobrachium lanchesteri) is most economically important and widely in distributed species in Thailand. There are consumed as traditional food, which are uncooked or cooked and processing in many forms; and lack of bacterial contaminant amount and antibiotic susceptibility pattern of each pathogen in M. lanchesteri were reported for surveillance of food safety and for consumer concerned. Aims of this study were conducted on 1) isolation and determination of bacterial contaminants; and evaluation of antibiotic susceptibility pattern in M. lanchesteri. Shrimp samples (N = 50) were purchased from ten of local markets in Bangkok and Nonthaburi, Thailand. Bacterial isolation and characterization were defined by colony morphology, Gram?s staining and biochemical tests. The level of identified bacteria in shrimps was determined by conventional plate count method. Antibiotic susceptibility of each bacterial isolate was determined by agar disc diffusion method (Kirby–Bauer test) and fifteen of standard antibiotic discs were used. Number of bacteria in M. lanchesteri shrimps were 151 isolates. Major pathogenic bacteria isolates were Aeromonas caviae, A. hydrophilla, Proteus penneri, P. vulgaris and Citrobacter freundii; and contamination was ranged within 1.8 × 107 to 1.2 × 108 CFU/ml. A. caviae was multi-drug resistance for ceftazidime, ampicillin, trimethoprim/sulfamethoxazole, tetracycline, cefuroxime, imipenem, gentamicin, and chloramphenicol. This finding was provided carried identification of pathogenic bacteria and antibiotic resistance data for surveillance of outbreak especially in raw or undercooked shrimp and its products consumers especially specific cultural style on food preparation.
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| Keyword:
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antibiotic resistance, bacterial contamination, foodborne pathogen, Macrobranchium lanchesteri, tiny freshwater shrimp.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.711
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| Download:
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