|
Anticancer Activity of Curcumin against Growth of Oral Squamous Cell Carcinoma
|
|
| Author:
|
A Y PRATAMA, I SUFIAWATI, R LESMANA, U SUPRATMAN
|
| Abstract:
|
Introduction: Oral Squamous Cell Carcinoma is the malignancy which commonly found in the oral cavity. Treatment of OSCC patients includes surgery, radiotherapy, and chemotherapy. The use of chemotherapy drugs such as cisplatin lead to many side effects and toxic effect for the patients, hence many natural ingredients that have anticancer activity are developed, such as curumin. Objective: To determine the anticancer activity of curcumin against OSCC and determine whether curcumin could be an adjuvant for chemotherapy drug cisplatin. Methods: Cal27 cell line (OSCC cell), which were purchased from American Type Culture Collection (ATCC), were cultured into 96 well plates. The method used was a cytotoxic test using PrestoBlue™ as a reagent with DMEM as a media control, cell + DMEM as a negative control, cisplatin as drug control, DMSO2% as solvent control, and curcumin, cisplatin, and curcumin in combination with cisplatin 5.3µM as the treatment group for which the dose was determined (100, 50, 25, 12.5, 6.25, 3.13, 1.56, 0.78 µg / mL). Anticancer activity was determined by calculating IC50 from the treatment group. Results: IC50 values of curcumin, cisplatin, and curcumin in combination with cisplatin 5.3µM were 27.67 µg/mL, 7.15 µg/mL, and 14.37 µg/mL, respectively. Conclusion: Curcumin potentially has anticancer activity against OSCC and could be used as an adjuvant treatment with cisplatin to reduce the toxicity and side effects produced by the chemotherapy drug cisplatin.
|
| Keyword:
|
OSCC, Anticancer, Curcumin, Cisplatin, IC50
|
| EOI:
|
-
|
| DOI:
|
https://doi.org/10.31838/ijpr/2021.13.01.710
|
| Download:
|
Request For Article
|
|
|