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Reversed Phase High Performance Liquid Chromatographic (Rp-Hplc) Method for Estimation of Tacrolimus in Bulk and Pharmaceutical Nanoparticles
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| Author:
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SNEHAL PATEL, CHINTAN AUNDHIA, KARTIK PANDYA
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| Abstract:
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An accurate, simple, and reproducible liquid chromatographic method was developed and validated for the
determination of Tacrolimus in nanoparticles. The analysis was performed at ambient temperature on a
reversed-phase C18 column with UV detection at 213 nm. The mobile phase was acetonitrile (100 %) used at
a constant flow rate of 1 ml/min. The method was validated in terms of linearity, precision, accuracy, and
specificity. The response was linear in the range of 200-1000 µg/ml (r2 = 0.9897). The relative standard
deviations for intra- and inter-day precision studies were found to be less than 2% which indicates method is
precise
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| Keyword:
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Tacrolimus, RP-HPLC, Acetonitrile, Validation, ICH.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.334
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| Download:
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Request For Article
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