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Assessment of Antibiotic Resistance to ß-Lactam in Staphylococcus Spp. Isolates
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| Author:
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AMER AHMAD AL-OMARI
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| Abstract:
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Staphylococci, which are among the most frequently isolated factors that are isolated from life-threatening nosocomial infections, have become a major health problem both in hospitals and community-acquired infections due to their increasing resistance to antibioticsSpecific antibiotic use favours the appearance of oxacillin-resistant strains of Staphylococcus spp. The resistance mechanism is caused by the development of the MacA-coded PBP2a protein. Any clinical isolates have the mecA gene but are susceptible to Oxacillin (OXN) and Cefoxitin (CFX) levels, that is, the mecA presence is not synonymous with its expression, in these isolates called pre-MRS or OS-MRS. It is thought that pre-MRS isolates will become immune when exposed to selective pressure due to the indiscriminate use of antibiotics. The purpose of this research was to determine whether the growth of oxacillin and CFX-resistant Staphylococcus strains was attributable to PBP2a (induction) or resistant strain antibiotic selection stimulation. 42 Staphylococcus spp. isolates, 25 Staphylococcusaureus, 17 Staphylococcus Coagulase Negative (SCN) of the Recife-PE University Hospital have been examined for this purpose. The MICs of the isolates were determined before an exposure to the antibiotic, Western blot and indirect immunofluorescence (IFI) tests and tolerance induction tests and antibiotic selection tests were conducted. PBP2a was recognised before an exposure was determined. Western blot and IFI revealed that PBP2a was also in the loss of antibiotics in some samples. The MIC stabilised and the isolates did not obtain a stability MIC, which imply a selection process, with the OXNinduction test. Variation between the colonies of the same isolate has been observed for testing to validate the selection mechanism, which characterises a hetero-resistance profile when some isolates is homogeneous with high resistance. These results affirm the relevance of mecA gene and PBP2a protein in clinical practise for carrying out antibiotic prescription.
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| Keyword:
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Antibiotic resistance, S. aureus, Cefoxitin,Oxacillin, mecA, western blot test,
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.370
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| Download:
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