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Amended homology model intended for T1R2 subunit of human sweet taste receptor using Phyre 2.0 and ModLoop
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| Author:
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SHOM PRAKASH KUSHWAHA, SUJEET KUMAR GUPTA, SUSHIL KUMAR
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| Abstract:
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Sugar substitutes help to control sugar blood in people with diabetes. The primary human receptor
responsible for sweet taste consists of two G-protein coupled receptor subunits, hT1R2 and hT1R3. Earlier
homology model of hT1R2 subunit has been reported and presence of outliers region was a drawback. We
developed an amended Homology Model for hT1R2 subunit of human sweet taste receptor using Phyre2 and
ModLoop. Amino acid sequence of hT1R2 receptor was salvaged from national center for biotechnology
information, U.S. national library of medicine. (Accession: Q8TE23.2, GI: 116242831). Amino acid sequence
was loaded into protein homology / analogy recognition engine V 2.0 and the generated model was subjected
to RAMPAGE Ramachandran plot analysis. Valuation specified 690 (89%) of residues existed in utmost
preferred region, 56 (7.2%) in allowed region and 29 (3.7%) in outliers region in hT1R2 model. The generated
model was repetitively exposed to loop alteration by means of Modloop web server and RAMPAGE
Ramachandran plot analysis. After twenty one cycles of loop refinement and analysis, an amended homology
hT1R2 model was obtained. It showed 723 (93.3%) residues in utmost favored area, 52 (6.7%) in permissible area and a total absence (0%) in outliers region.
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| Keyword:
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hT1R2, homology model, ModLoop, Phyre2, RAMPAGE.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.292
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| Download:
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