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Histopathology And Genetics Study Of Sperm Abnormalities In Mice Infected With Brucella Meletensis
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| Author:
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MUNA SACHIT HASHIM, THIKRA ABDULLAH MAHMOOD, EMAN HASHIM YOUSIF
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| Abstract:
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Background: Mammalian spermatogenesis is a process that includes the propagation and differentiation in seminiferous tubules of the testis cell. It’s characterized by meiotic, mitotic divisions and spermatogenesis. Exogenous and endogenous chemical, physical, and biological causes modify the genome of spermatozoa. Marinating of the genomic stability and integrity of the sperm is a vital event and it is regulated by DNA repair processes; DNA repair processes in male germline involving base excision repair, nucleotide excision repair, DNA mismatch repair, double-strand break repair and post-replication repair. It defects in repair processes cause the arrest of spermatogenesis and abnormal recombination, eventually causing male infertility.
Methodology: In this study, about 25 mice were experimentally infected with Brucella melitensis to investigate the pathological alterations on testicular tissues, as well as for detection of DNA damage of daily repairing gene 22q11.2 in Spermatocytes using the FISH technique.
Results: samples showed frequently of DNA damage appear as deletion at 22q11.2 gene of spermatocytes. Histopathological examination of mice infected with Brucella melitensis revealed necrotic orchitis with obvious loss of spermatogenesis within seminiferous tubules associated with a severe inflammatory response.
Conclusion: genetic regulation of DNA repair process in spermatocytes was losing in brucellosis owing to damage in chromosomal material at gene locus responsible for DNA regular repairing process of the sperm, as well as testicular tissues damage.
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| Keyword:
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Brucellosis, DNA damage, FISH technique, Spermatogenesis
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.03.487
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| Download:
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