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Characterization of Carotenoids from Bacterial Symbiont Virgibacillus salarius Strain 19.PP.Sc1.6 from Panjang Island, Jepara, North Java Sea Indonesia
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| Author:
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LIA KUSMITA, OCKY KARNA RADJASA, AGUS SABDONO, AGUS TRIANTO
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| Abstract:
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Carotenoids are secondary metabolites commonly found in organisms on land and at sea. One of the marine organisms producing carotenoid compounds is soft corals, a slow growth and delicate group that its exploiting can damage the balance of the reef ecosystem. The use of symbiotic bacteria of soft corals is an alternative that can be developed in a sustainable way. The purpose of this research is to find out the characterization of symbiotic carotenoid-producing bacterium Virgibacillus salarius strain 19.PP.Sc1.6 of softcoral Sinularia sp. Samples of softcoral were obtained from Panjang Island, Jepara, North Java Sea, Indonesia. Carotenoid identification method was carried out by using a spectrophotometer UV-Vis, TLC, and HPLC. Antioxidant activity test used the DPPH method. Sunscreen activity was performed by the Mansyur method using a UV spectrophotometer-Vis. The results revealed that bacterial symbiont V. salarius strain 19.PP.Sc 1.6 contains carotenoids, one of which was nostoxantin. Carotenoid extract had antioxidant activity with value IC50 of 438 ppm. Carotenoid extract at a concentration of 438 ppm had an SPF value of 4. 97, and was considered within the medium protection category, with Te value of 29.91% and% Tp of 21.34% in the UVA sunblock category, respectively.
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| Keyword:
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carotenoids, symbiotic bacteria, antioxidants, sunscreen
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.274
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| Download:
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