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New Method Development to Isolate Andrographolide Compound from Andrographis Paniculata (Burm. F) Nees Using Al2O3
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| Author:
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, KURNIA RAHAYU PURNOMO SARI, NOFRAN PUTRA PRATAMA, RIZQA SALSABILA FIRDAUSIA
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| Abstract:
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This study aimed to determine the effect of using Al2O3 active neutral in the process of andrographolide
isolation from Andrographis paniculata. The extraction of Andrographis paniculata herbs was carried out with
ethanol solvent. Isolation of andrographolide was continued using non-polar solvent, then passed to Al2O3
column and eluted with methanol. Next, the purification of isolated crystal was obtained by recrystallizing the
extract using cold methanol. Identification of the isolated crystal was confirmed qualitatively by an analysis of
the melting point, TLC, and FTIR spectra. The data obtained then compared with an analytical grade of
andrographolide as the standard. The results showed that the data of isolated crystal was similar to the
andrographolide standard from the parameter of melting point, TLC analysis, and FTIR spectra. From this
research is known that isolation of andrographolide using Al2O3 could be an alternative method which more
efficient and less solvent. In conclusion, this method is potentially developed as one of the methods to isolate
andrographolide compounds from Andrographis paniculata
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| Keyword:
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andrographolide, Al2O3, isolation, characterization
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.255
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| Download:
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Request For Article
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