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Method Development and Validation of Glucosamine in Marketed Tablet Using RP-HPLC Method
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| Author:
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LODOE CHOEZOM, R.S.CHANDAN, GURUPADAYYA BM, BARATH M
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| Abstract:
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Glucosamine is a popular dietary supplement for the treatment of osteoarthritis and other joint pain. Analysis
of glucosamine is challenging due to the absence of UV absorbing chromophore or the fluorescence
properties, therefore it is derivatized to incorporate chromogenic or fluorogenic compound before the
analysis. In the present work, a simple precolumn derivatization liquid chromatography was developed and
validated for the analysis of glucosamine in a marketed tablet. OPA (o-phthaldialdehyde) reagent was used to
derivatize the glucosamine into a fluorescence compound. The HPLC separation of the derivative is achieved
on the Phenomenex Luna C18 column (150 mm × 4.6 mm, i.d., 5 m particle size) at flow rate of 0.9 ml/min of
mobile phase as acetonitrile and 0.25% THF (tetra hydro furan) in Millipore water in a ratio of 70:30 v/v and
fluorescence detection with excitation ?max of 335nm and an emission ?max of 445nm. The glucosamine is
eluted at the retention time of 2.9 minutes. The regression coefficient obtained was 0.9936 indicating excellent
linearity. The limit of detection and limit of quantification are 0.014µg/ml and 0.577µg/ml respectively. The
content of glucosamine in the pharmaceutical marketed tablet was found to be 97.49%. The developed method
was found to be simple, fast, reliable, accurate, precise, and can be used for analysis of glucosamine in other
formulations as well.
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| Keyword:
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Glucosamine, osteoarthritis, Derivatisation, HPLC, Validation
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.253
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| Download:
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