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Title: Association of p53 and p21 gene polymorphism and risk of gastrointestinal cancer in rural population of south western Maharashtra: A hospital based case-control study.
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| Author:
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MADHAVI N.PATIL, ANAND K.GUDUR, RASHMI A.GUDUR, SANDEEP KADAM, PARIXIT BHANDURGE, SURAJ PAWAR, SATISH KAKADE, KAILAS D.DATKHILE
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| Abstract:
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As compared to the activation of oncogenes, the mutations in the tumour suppressor genes may lead to the loss of function for these genes and promote the development of cancers in humans. In this regard the polymorphism of p53 and p21 gene is one of the major research focus worldwide. This intended us to study the association between genetic polymorphism in tumour suppressor gene (p53 and p21) and gastrointestinal cancer (GI) risk in the rural population of south-western Maharashtra. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 200 untreated GI cancer patients and 400 age & sex matched controls, to study the polymorphism in p53 & p21 gene and their association with GI cancer risk. Our results revealed no significant association for GI cancer risk with the polymorphism in exon 2 of p21(OR:1.20; CI:0.58-2.47; p:0.61) & exon 3 of p21(OR: 1.52; CI: 0.70-3.30; p=0.28) and exon 4 of p53(OR: 0.94; CI: 0.59-1.50; p: 0.81) genotype. However, statistically significant association was observed between codon 249 of exon 7 in p53 gene (OR: 1.96; CI: 1.25-3.07; p=0.003) with development of GI cancer. Our findings from this study conclude that there is a possible correlation between gastrointestinal cancer risk and polymorphism in codon 249 of exon 7 of p53gene in South Western Maharashtrain population.
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| Keyword:
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Geneetic polymorphism, Gastrointestinal Cancer, p21, p53, PCR-RFLP.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.168
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| Download:
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