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Effect of Kinetic Parameters on L-Asparaginase from Streptomyces Albogriseolus
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| Author:
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MITHILESH KUMAR, VENKATESH KAMATH B, MRINALINI R, ARAVINDA PAI, BHAVANA B BHAT, ASWATHA RAM H N
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| Abstract:
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L-Asparaginase is an essential entity in treating acute lymphoblastic leukemia and other types of Blood
Cancers. The inhibitory action of the L-Asparaginase on the synthesis of Proteins associated with tumor cells,
makes it a very sought after first-line treatment choice for acute lymphoblastic leukemia and associated
diseases. L-Asparaginase is processed by a varied range of source including animals, plants and microorganisms,
where this article focuses on the microorganisms producing a substantial quantify of the enzyme. The novel
microorganism of interest is Streptomyces albogriseolus, which is an aerobic, Gram-positive bacteria. This
bacterial strain is found to secrete a broad spectrum of chemicals such as L-Asparaginase, eunicellin
diterpenoids and various alkaloids to name a few. The L-Asparaginase isolated from Streptomyces albogriseolus is
subjected to different test conditions to detect the ideal conditions for the maximum enzymatic activity. The
parameters considered for the ideal conditions were substrate (L-Asparagine) concentration, pH and
temperature. The test results showed that 0.04M substrate concentration, pH 8.0, and 40ºC temperature
were the optimal test conditions under each parameter taken.
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| Keyword:
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Streptomyces albogriseolus, substrate concentration, pH, temperature, L-Asparaginase.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.210
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| Download:
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