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RP-HPLC Method Development and Validation for the Estimation of Cefpodoxime Proxetil in Bulk and Pharmaceutical Dosage
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| Author:
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SHAIKH SIRAJ N, SANDEEP S KSHIRSAGAR, G J KHAN, PATEL M SIDDIK N, M Y KHALIFA, MAKRANI SHAHARUKH I, ANSARI YASIR A RAZZAQ, JAYESH C CHAUDHARY
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| Abstract:
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The objective of present work was to develop and validate a simple, accurate, precise RP-HPLC method for
the estimation of Cefpodoxime Proxetil in Bulk and Pharmaceutical Dosage Tablet form. The separation was
done by using Agilent technology RP-HPLC .Column Agilent C18 (4.6 x 150 mm, Particle size 2.5 ) with
Mobile Phase was Methanol : 0.1 % Ortho phosphoric acid ( OPA) Water pH -4.2 with Triethylamie
(20:80% v/v). The flow rate was 0.7 ml/min, and the analytes were measured at ?max 236 nm. The retention
time for Cefpodoxime Proxetil was 4.864 min, respectively. The method was validated with respect to system
suitability, linearity, precision, accuracy, and specificity, limit of detection (LOD), limit of quantification (LOQ),
robustness and ruggedness. Linearity studies was performed (5–25µg/ml), The LOD of Cefpodoxime Proxetil
was determine at 0.1579 and LOQ 0.4786 is respectively. These data show that method is sensitive for the
determination of Cefpodoxime Proxetil.The relative standard deviation for accuracy was found to be less than
2%. The mean percentage recovery was 101.08 to 99.97% . The results of recovery studies indicate that the
proposed method is highly accurate. The proposed validated method was successfully applied to determine
Cefpodoxime Proxetil in tablet dosage form. The proposed method shall prove equally effective to analyze
Cefpodoxime Proxetil in the corresponding drug sample and may prove to be of great importance in
pharmaceutical analysis
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| Keyword:
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Cefpodoxime Proxetil, Methanol, Ortho phosphoric acid, Method Development, Linearity, Precision.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.160
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| Download:
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