|
The effect of green tea extract (Camellia sinensis L. Kuntze) and equilibration period difference on post-thawed Kacang buck (Capra hircus) semen quality
|
|
| Author:
|
SUHERNI SUSILOWATI, IMAM MUSTOFA, WURLINA WURLINA, TATIK HERNAWATI, YUDIT OKTANELLA
|
| Abstract:
|
Kacang goats (Capra hircus) are an indigenous Indonesian livestock species that can be reared by local communities as an additional source of income to prevent poverty and to provide animal protein. Artificial insemination techniques could use to increasing the population size, preserving genetically valuable offspring, and improving reproductive performance. This study was the first to combine the antioxidant addition in skim milk–egg yolk (SM–EY) extender and different equilibration period to obtain a higher quality post-thawed Kacang buck semen. The present study aimed to determine the effect of green tea (Camellia sinensis L. Kuntze) extract (GTE) on the quality of frozen Kacang goat sperm equilibrated for 1 and 2 h. The pool of Kacang buck ejaculate was divided into four portion equally and diluted in a SM–EY extender that contained four doses of 0, 0.05, 0.10, and 0.15 mg of GTE/100 mL for T0, T1, T2, and T3 groups, respectively. The aliquots were treated with a 1 or 2 h equilibration period before further processing as frozen semen. Post-thawed semen's quality was evaluated for sperm viability, progressive motility, intact plasma membrane, malondialdehyde concentration, and DNA fragmentation. The results showed that post-thawed semen of T2 was the highest quality than other groups. Meanwhile, 1 h equilibration period had better quality than that of 2 h equilibration. It could be concluded that a combination of 0.1 mg GTE/100 mL extender as an antioxidant and 1 h equilibration period provided the best result for frozen Kacang buck semen.
|
| Keyword:
|
artificial insemination, DNA fragmentation, equilibration, indigenous goat, IPM, MDA, motility, prevent poverty, viability
|
| EOI:
|
-
|
| DOI:
|
https://doi.org/10.31838/ijpr/2021.13.02.214
|
| Download:
|
Request For Article
|
|
|