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Anticariogenic And Anticarcinogenic Effects Of Garcinia Mangostana Pericarp Extracts On Cariogenic Bacteria And On Cancer Cell Lines With Molecular Docking Study
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DR.SUNITHA JANARDHANAN, DR.JAIDEEP MAHENDRA, DR. REVATHI KASTHURI, DR. LITTLE MAHENDRA, DR.SRUTHI SRINIVASAN, DR. SAJID. T. HUSSAIN
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| Abstract:
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AIM: The present study explored the role of mangosteen pericarp extract on oral cariogenic organisms, anticarcinogenic potential on oral cancer and cervical cancer cell lines grown in-vitro, role of alpha mangostin, an active component of mangosteen pericarp on genes commonly expressed in oral cancer using molecular docking technique. When the ecology of the microbiome is disturbed it results in chronic inflammation. This chronic inflammation leads to genetic changes and finally cancer. Herbs like mangosteen have shown tremendous potential as an antibacterial and anticancer agent in numerous studies.
METHODOLOGY: The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values were also determined. The anticancer potential of the crude ethanolic extract of mangosteen against two cancers - oral cancer cells and cervical cancer cells grown in vitro were also investigated. The viability of the cancer cells was assessed by doing a MTT assay, indicating the cytotoxic potential of mangosteen. The anticarcinogenic action was further assessed by the ability of mangosteen to cause apoptosis in cancer cell lines by TUNEL assay, Annexin/PI flow cytometry and western blot analysis. In addition, this study explored the inhibitive role of alpha mangosteen on genes commonly expressed in oral cancer using molecular docking technique.
RESULTS The results highlight the fact that mangosteen is effective as an anticariogenic against Streptococcal species of microorganisms and anticarcinogenic agent. The pericarp showed promising results as an anticancer agent by inducing apoptosis in both oral cancer and cervical cancer cell lines. CONCLUSION The present study showed that the key component of the pericarp, alpha-mangostin could inhibits the genes CALM3 and HTT and had the potential to be used as a targeted therapy on oral cancer. Moreover, mangosteen can play a key role in preventing oral dysbiosis by inhibiting the organisms and by causing apoptosis in cancer cells.
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| Keyword:
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Mangosteen, pericarp, cell lines, anticariogenic, extracts, molecular docking.
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.040
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