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Optimization of 4-Hydroxychalcone Gel Formulation and Determination of UV-B Absorption Sunscreen Activity by In Vitro and In vivo
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| Author:
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, RURI PUTRI MARISKA, ABDUL KARIM ZULKARNAIN, RINA KUSWAHYUNING
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| Abstract:
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4-hydroxychalcone is a chalcone derivative that can be an antioxidant and can thus be used as an active
ingredient in sunscreen UV-B absorption. This research aimed to determine the levels of carbopol, CMC-Na,
and gelatin that could generate the optimum formula for 4-hydroxychalcone gel, to test the physical
properties, and to determine the behavior of UV-B absorption of sunscreen in vitro and in vivo. We calculated
the optimum formula on the physical properties of the gel using the Simple Lattice Design method with
Design-Expert version 10.1 software. Data analysis with a one-sample t-test was performed to determine the
variations in the results of the physical properties test of the optimum formula generated in the software and
the results of the observations. Sunscreen activity was seen from the SPF value collected from in vitro UV-Vis
spectrophotometer and in vivo tests using New Zealand White female rabbits. The findings revealed that the
proportion of carbopol, CMC-Na, and gelatin that provided the optimum formula for 4-hydroxychalcone gel
was 1%; 2.5 %; and 1.5 %. Physical properties such as dispersion forces, adhesion, and viscosity of the results
observations in the software did not vary significantly. The in vitro activity of 4-hydroxychalcone sunscreen
gave a Sun Protection Factor (SPF) value of 21.49 and in vivo resulted in an SPF value of 21. The optimum
formula for 4-hydroxychalcone gel preparation had moderate UV-B absorption sunscreen activity.
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| Keyword:
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gel, 4-hydroxychalcone, optimization, sun protection factor
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.155
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| Download:
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