Morphological aspects of the spleen of white mongrel rats after severe traumatic brain injury caused experimentally in the form of a road accident
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Author:
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, XURSHID B. FAYZIEV, NIGORA K. KHAMIDOVA, SHUKHRAT J. TESHAYEV, SALIM S. DAVLATOV
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Abstract:
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The study was conducted on 81 white 3-month-old mongrel rats. Animals were fixed with a device made by
hand in the form of a vehicle on wheels, the fixed rats were accelerated on the vehicle and hit the wooden
barrier with the frontal part of the head. As a result of this experiment, 16 white rats died on the spot. All the
rats that died during the experiment were decapitated on the spot by means of instant decapitation of the
animals. After opening the abdominal cavity, the spleen was removed. The removed spleen was examined and
examined and its size was measured in accordance with the established procedure. The spleen was fixed in
Buena solution and poured into paraffin according to generally accepted rules. Next, histological sections were
prepared with a thickness of 6-7 microns, which were stained with hematoxylin-eosin and van gieson.
Morphometric studies of spleen tissue were performed under the NLCD-307B microscope. In 3-month-old
rats, the total thickness of the spleen wall in its various ranges remained unchanged, but some changes in the
tissue structures of the white and red spleen pulp were detected.
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Keyword:
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rat, spleen, microanatomy, red pulp, white pulp.
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EOI:
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DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.151
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