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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Morphological aspects of the spleen of white mongrel rats after severe traumatic brain injury caused experimentally in the form of a road accident

Author: , XURSHID B. FAYZIEV, NIGORA K. KHAMIDOVA, SHUKHRAT J. TESHAYEV, SALIM S. DAVLATOV
Abstract: The study was conducted on 81 white 3-month-old mongrel rats. Animals were fixed with a device made by hand in the form of a vehicle on wheels, the fixed rats were accelerated on the vehicle and hit the wooden barrier with the frontal part of the head. As a result of this experiment, 16 white rats died on the spot. All the rats that died during the experiment were decapitated on the spot by means of instant decapitation of the animals. After opening the abdominal cavity, the spleen was removed. The removed spleen was examined and examined and its size was measured in accordance with the established procedure. The spleen was fixed in Buena solution and poured into paraffin according to generally accepted rules. Next, histological sections were prepared with a thickness of 6-7 microns, which were stained with hematoxylin-eosin and van gieson. Morphometric studies of spleen tissue were performed under the NLCD-307B microscope. In 3-month-old rats, the total thickness of the spleen wall in its various ranges remained unchanged, but some changes in the tissue structures of the white and red spleen pulp were detected.
Keyword: rat, spleen, microanatomy, red pulp, white pulp.
DOI: https://doi.org/10.31838/ijpr/2021.13.02.151
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