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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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A Colorimetric Analysis Study of Uric Acid in Goat Patients

Author: ABBAS SHEBEEB HASAN AL-KADUMI, NOOR MAZIN IBRAHIM, DUHA BAHAA MOHAMMED AL-FAYAAD
Abstract: Simple, sensitive, precise and accurate colorimetric method for the analysis of uric acid was proposed. The method was based on the complexing reaction between uric acid with potassium hexacyano ferrate and ferric chloride in a hydrochloric acid medium to form Prussian blue color complex, the optimum conditions were (maximum wavelength was 735 nm, volume 0.3 ml for hydrochloric acid,1.25 ml for both Potassium hexacyano ferrate and ferric chloride solutions, 60 ºC and 8 minutes as maximum temperature and time for the reaction, the order of mixing additives was uric acid, ferric chloride, Potassium hexacyano ferrate and hydrochloric acid). The highly accurate and precise results obtained with RSD%, Recovery%, Ere% and D.L were (0.755 to 4.376), (99.676 to 97.831), (-0.033 to -2.169), and 0.1569 mg.L-1, respectively. The linearity was in the range between (2.0-200 mg.L-1). The proposed methods were successfully applied for the analysis of uric acid in serum and urine samples for twenty persons
Keyword: Uric acid, colorimetry, analysis, gout patients
DOI: https://doi.org/10.31838/ijpr/2021.13.02.153
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