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Physalis Angulata Leaf Extract Protects Against Oxidative Stress and Antiangiogenic Factor in LNAME-Induced Preeclampsia Rats
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| Author:
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DIAN NUGRAHENNY, DR SETYAWATI SOEHARTO, DR NUR PERMATASARI, ACHMAD RUDIJANTO, DR I WAYAN ARSANA WIYASA, EDI WIDJAJANTO, MOHAMMAD ARIS WIDODO, DR KARYONO MINTAROEM, ELLY MAYANGSARI
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| Abstract:
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Objectives
This study evaluated the protective effect of Physalis angulata leaf extract on L-NG-nitroarginine methyl ester
(L-NAME)-induced preeclampsia rats.
Methods
Twenty-five pregnant Wistar rats were randomly divided into five groups. Preeclampsia rats were injected with
L-NAME on the 5th to 17th day of gestation (G5-G17), while sham rats were injected with the same vehicle
volume. Preeclampsia rats were administered with Physalis angulata leaf methanol extract at 500, 1500, or 2500
mg/kg body weight/day on G1-G17. The systolic blood pressure measurement was performed by the tail-cuff
method. Placental VEGFR2 expression was determined by immunohistochemistry. The serum sFlt-1 level was
evaluated using ELISA. Serum MDA level, SOD activity, and total nitrite level were measured by colorimetry.
Results
The L-NAME injection increased systolic blood pressure (1.44-fold), serum sFlt-1 level (1.22-fold), and serum
MDA level (2.03-fold). It also decreased placental VEGFR2 expression (1.18-fold), serum SOD activity (2.11-
fold), and serum nitrite level (1.25-fold) compared to the Sham group. The extract supplementation prevented
the rise in systolic blood pressure and serum sFlt-1 level. It also tended to inhibit the reduction in the placental
VEGFR2 expression. It prevented the increase in serum MDA level. It also prevented the decrease in serum
SOD activity and nitrite level.
Conclusions
We concluded that Physalis angulata leaf methanol extract inhibits oxidative stress and an antiangiogenic factor
in the rat preeclampsia model. These findings support that the extract has the potential to prevent
preeclampsia development
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| Keyword:
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Antioxidant; Cutleaf groundcherry; High blood pressure; Nitric oxide; Pregnancy; Soluble fms-like tyrosine kinase 1
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.577
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| Download:
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