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Characterization and Optimization of Biogenic Silver Nanoparticles of Ananas Comosus Peels for Antimicrobial Synergy Against Pathogenic Microbes
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| Author:
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VIKRAM KUMAR, UMESH KUMAR GILHOTRA, SUDARSHAN S LAKHAWAT, SUNIL KUMAR, SATISH KUMAR
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| Abstract:
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Nanoparticles fabrication is done by using Green synthesis because of its large applications in different field
such as pharmaceutical, biomedical, food and agriculture engineering. In this paper pineapple peel extract is
taken that contain alkaloids, flavonoids, saponins, steroid compounds, acts as reducing and stabilizing agents. In
this paper AgNPs of pineapple peel were used to confirm their antimicrobial screening, because pineapple peel
contains alkaloids, flavonoids, saponins, steroid biomolecules which helps in nanoparticle biosynthesis by
playing the role of reducing and stabilizing agents. The characterization was completed by using the UV-visible
spectrophotometer, FTIR and SEM techniques and the method optimization was done by varying the peel
extract volume, silver nitrate concentrations, pH and temperature. Antimicrobial activity was evaluated by the
Zone of inhibition method. The biosynthesized AgNPs produced significant antimicrobial activity against
pathogenic Staphylococcus aureus, Enterococcus faecalis (a Gram-positive bacterium), Pseudomonas aeruginosa and
Escherichia coli (a gram negative bacterium) and Aspergillus niger (plant pathogenic fungal strain), Candida albicans
(human pathogenic fungal strain). Henceforward, this research can be acknowledged in future because silver
nanoparticles of pineapple peel extract have potential significant antimicrobial activity.
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| Keyword:
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Silver Nanoparticles; AgNPs; Pineapple peel; Antimicrobial; Bacteria
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.099
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| Download:
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