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Development of a New Methodology for Amlodipine Besylate (ADB) Determination Using Potassium Hexacyanoferrate (III) (PHF) as a Reagent and ISNAGFluorimeter Instrument via CFIA
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| Author:
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NAGHAM S.TURKEY AL AWADIE, GHUFRAN KHALID
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| Abstract:
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A new, simple, fast and sensitive method has been developed for the determination of Amlodipine besylate
pure form and drugs (tablets) by continuous flow injection scatter light. The method was based on the
reaction of the Amlodipine besylate with Potassium hexacyanoferrate (III) to form a precipitate, using
homemade ISNAG- Fluorimeter. Optimum parameter have been studied to increase the sensitivity for
developed method. The scatter plot range and the linear dynamic range for the instrument response versus
Amlodipine besylate concentration was 0.1- 45 mmol/L while the L.O.D was 3.984µg /sample from the step
wise dilution for the minimum concentration of lowest concentration in the scatter plot range of the
calibration graph. The correlation coefficient (r) was 0.9849 and 0.9987 while percentage linearity (R2%) was
97% and 99.75 for the scatter plot range and the linear dynamic range(respectively). RSD% for the repeatability
(n=8) was lower than 0.6% for the determination of Amlodipine besylate, with concentration of 3, 10 mmol/L
respectively. The methods: developed method was applied successfully for the determination of Amlodipine
besylate in pharmaceutical tablets. A comparison was made between the newly developed method with the
classical method (Turbidemtric method and UV-spectrophotometer method) of analysis using the standard
addition method via the use of paired t-test. It shows that there was no significant difference between the
quoted values of each individual company with calculated t-value at 95% confidence interval from developed
method.
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| Keyword:
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Amlodipine besylate, flow injection, scatter light, ISNAG-Fluorimeter instrument
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.091
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| Download:
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