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Association of Single Nucleotide Polymorphism (SNP) in Exon 4 IGF-1 Gene with Sperm Quality in Bali Bulls
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| Author:
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MASKUR , MUHAMMAD MUHSININ, TAPAUL ROZI, MADE SRIASIH, CHAIRUSSYUHUR ARMAN, AHMAD FUDHOLI
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| Abstract:
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Sperm Quality is one of the primary traits of reproduction in bulls. Insulin-like growth factor-1 (IGF-1) gene is
one of the markers candidates for sperm quality and fertility in bulls. This study was conducted to determine
the effect of the new single nucleotide polymorphism (SNP) in exon 4 IGF-1 gene to sperm quality in Bali bulls.
A total of 164 bulls were genotyped for RsaI restriction site in the exon 4 of IGF-1 by applying PCR-RFLP
method. Semen samples were collected to analyze the characteristics of semen namely sperm volume,
concentration, viability and motility. The results of this study showed the presence of SNP in exon 4 IGF-1
gene that caused by the C/T transition. Genotyping of Bali bulls on exon 4 IGF-1 genes produced two alleles,
namely C and T alleles with the allele frequencies were 0.793 and 0.207, respectively and three individual
genotypes namely TT, CT and CC with successive frequencies: 0.170; 0.074; and 0.756. Polymorphism of IGF-
1/Rsa1 genotype has significantly effect the semen quality of Bali bulls
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| Keyword:
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IGF-1, restriction, SNP, semen quality and Bali bulls
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.619
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| Download:
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