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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Anti-Salmonella Products from Immobilized Marine Bacillus Amyloliquefaciens AMM

Author: , ABDUSALAM F.A.OMAR, MANAL M.EL-NAGGAR, DALIA TALAT, HOSAM SALEH, MADIHA S.IBRAHIM
Abstract: Marine microorganisms are conceded as a rich source for bioactive metabolites. However, the microbial diversity of marine ecosystem still needs to be explored. The aim of this study was to isolate and characterize a marine bacterium with antimicrobial activities. Samples of seawater, sediment and algae were collected from the marine coast of Alexandria, Egypt. Twenty two isolates were screened for their ability to produce antimicrobial agent(s). Four isolates from sediment samples showed antibacterial activity against Salmonella enterica. Based on morphological characterization, biochemical and molecular examination, the potent antimicrobial producer was identified as Bacillus amyloliquefaciens AMM. It showed inhibition zone of 10mm on optimized marine nutrient broth culture medium, The immobilized process was performed using the pumice stones it increased the antimicrobial activity twice (20mm) compared to the free cells (10mm). The n-Butanol secondary metabolites of the marine Bacillus amyloliquefaciens AMM (125ppm) showed extensive antibacterial activity against different pathogenic bacteria; Vibrio parahemolyticus Staphylococcus aureus, Pseudomonas aerogenosa, Escherichia coli and Salmonella enterica the inhibition zones were10, 7, 12, 14 and 24mm, respectively. The biotoxicity test of the produced anti-salmonella agent using Artemia salina showed a LC50 of 794.3 ppm. The GC-MS analysis of this study showed the main chemical components of the n-butanol extracted secondary metabolites of Bacillus amyloliquefaciens AMM were Cholestan-3-ol-2-methylene-, (3á,5à)-, Cyclopentanemethylamine, 2-isopropylidene-N,N,5-trimethyl , 1,2,3-Propanetriol-Triacetate , Formic acid- 3,7,11-trimethyl-1,6,10-dodecatrien-3 ester and 9-Octadecenoic acid with about 80% of the total area. In addition, the FTIR analysis showed the presence of many active functional groups in these obtained secondary metabolites.
Keyword: marine – natural product – Bacillus amyloliquefaciens - Salmonella sp. – immobilization
DOI: https://doi.org/10.31838/ijpr/2021.13.02.065
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