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Anti-Salmonella Products from Immobilized Marine Bacillus Amyloliquefaciens AMM
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| Author:
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, ABDUSALAM F.A.OMAR, MANAL M.EL-NAGGAR, DALIA TALAT, HOSAM SALEH, MADIHA S.IBRAHIM
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| Abstract:
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Marine microorganisms are conceded as a rich source for bioactive metabolites. However, the microbial
diversity of marine ecosystem still needs to be explored. The aim of this study was to isolate and characterize
a marine bacterium with antimicrobial activities. Samples of seawater, sediment and algae were collected from
the marine coast of Alexandria, Egypt. Twenty two isolates were screened for their ability to produce
antimicrobial agent(s). Four isolates from sediment samples showed antibacterial activity against Salmonella
enterica. Based on morphological characterization, biochemical and molecular examination, the potent
antimicrobial producer was identified as Bacillus amyloliquefaciens AMM. It showed inhibition zone of 10mm on
optimized marine nutrient broth culture medium, The immobilized process was performed using the pumice
stones it increased the antimicrobial activity twice (20mm) compared to the free cells (10mm). The n-Butanol
secondary metabolites of the marine Bacillus amyloliquefaciens AMM (125ppm) showed extensive antibacterial
activity against different pathogenic bacteria; Vibrio parahemolyticus Staphylococcus aureus, Pseudomonas
aerogenosa, Escherichia coli and Salmonella enterica the inhibition zones were10, 7, 12, 14 and 24mm,
respectively. The biotoxicity test of the produced anti-salmonella agent using Artemia salina showed a LC50 of
794.3 ppm. The GC-MS analysis of this study showed the main chemical components of the n-butanol
extracted secondary metabolites of Bacillus amyloliquefaciens AMM were Cholestan-3-ol-2-methylene-, (3á,5à)-,
Cyclopentanemethylamine, 2-isopropylidene-N,N,5-trimethyl , 1,2,3-Propanetriol-Triacetate , Formic acid-
3,7,11-trimethyl-1,6,10-dodecatrien-3 ester and 9-Octadecenoic acid with about 80% of the total area. In
addition, the FTIR analysis showed the presence of many active functional groups in these obtained secondary
metabolites.
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| Keyword:
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marine – natural product – Bacillus amyloliquefaciens - Salmonella sp. – immobilization
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.065
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| Download:
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