Mycodiesel Production from some Isolates of Oleaginous Fungi Isolated from Oil-Rich Soils in Basrah
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Author:
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, INAAM MAHMOOD ALRUBAYAE, KADHIM FADHIL KADHIM
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Abstract:
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The present study was aimed to determine of mycodiesel production from oleaginous fungal isolates that included
Aspergillus niger, A.flavus, A.ochraceus, and Penicillium chrysogenum, three different fermentation media were used to
cultivate fungal isolates that included nitrogen limiting medium NLM, chees why medium CWM, and waste molasses
dates medium WMDM, the accumulation lipids of drying biomass were extracted by using 1:2 methanol: chloroform.
The result of biomass and lipids production were showed that NLM and CWM represented the best media for
accumulation of lipids and yield high biomass, while A. niger and P.chrysogenum were accumulated high percentage of
lipids by 36% and 38% respectively. Furthermore, the analysis of fatty acid methyl ester FAMEs was used by
transesterification reactions of extracted lipids. Subsequently, the FAMEs were analyzed by using GC/MS then Cetane
Number (CN) of mycodiesel was calculated, therefore, the results were showed that the percentage of the
composition of FAME ranged between 66.06 - 92.29 % , while the results of CN was 52.6 - 57. On the other hand the
results of residual carbon and sulfur content of mycodiesel product were 0.04 and 0.0069 wt% respectively
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Keyword:
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Mycodiesel , Oleaginous fungi , Oil-rich soils
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EOI:
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-
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DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.640
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