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Study the Effects of Green Tea (camellia sinensis) on Serum Malondialdehyde and Glutathione Concentration on High Cholesterol Fed Rats
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| Author:
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SUAAD M.J.AL-HADRAWY, NAJAT MUTAR ORAIBI AL SALAMI
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| Abstract:
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Background and Aim: In recent years great interest has emerged in studying the health benefits of green tea particularly in relation to cardiovascular disease and cancer. We therefore evaluated health aspects of green tea on the body antioxidant and oxidative stress biomarkers also their special effects on body weight and body weight gain under prolonged high cholesterol diets in White rats.
Materials and Methods: Twenty female albino White rats were randomly divided into four groups. Each group was fed with a different feeding system; each group consisted of five animals. Animals were fed for 10 wk. The four groups included a control (group 1) were fed regular pellet, the second group (group 2) were nourished with high cholesterol diet (3 % egg yolk and 4% coconut oil), the third group (group 3) were treated with green tea (0.6 ml of 5% green tea) orally for five times per week along with high cholesterol diet (3 % egg yolk and 4% coconut oil), and the fourth group (group 4) treated daily with green tea (0.6 ml of 5% green tea) orally for five times per week along with standard pellet diet. Blood samples were taken after 10 wk of nutritional treatment by cardiac puncture technique for estimated of serum glutathione (GSH), malondialdehyde (MDA), total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL-C), low density lipoprotein (LDL-C), very low density lipoprotein (VLDL-C) and also weight tests were done include body weight before and after experiment and body weight gains.
Results: Serum malondialdehyde (MDA) increased significantly (P?0.05) in group 2 (CH) after weeks 10 compared with other groups, also serum MDA significantly decrease in group 3 (CH + green tea) and group 4 (green tea). Serum glutathione concentration significantly increases (P< 0.05) in group 3 (CH + green tea) and group 4 (green tea) and there was significantly decrease (P? 0.05) in cholesterol group (group 2). Total cholesterol, TG, LDL-C and VLDL-C increased significantly (P? 0.05) in groups 2 (CH) after weeks 10 compared other groups also there were significant increase in the levels of HDL-C after 10 weeks in green tea group rats. Body weights increase (p?0.05) after experiment in cholesterol group. Body weights gain after experiment was a significantly increase (p?0.05) in cholesterol group, and there was a significant decrease (p<0.05) in body weights after experiment in animals treated with cholesterol+ green tea (group 3) and green tea group (group 4) matched with control and cholesterol group.
Conclusions: These data indicate that green tea supplementation resulted in elevated serum glutathione and decrease serum malondialdehyde. The green tea reduced the blood lipid and body weight gain.
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| Keyword:
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camellia sinensis; malondialdehyde; glutathione; high cholesterol diets.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.03.507
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| Download:
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