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Article Detail
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Molecular analysis of genetic elements responsible for drug resistance Proteus mirabilis isolated from Baghdad city hospitals
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| Author:
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RAWA ABDUL REDHA AZIZ, SURA ALAA SAUD
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| Abstract:
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Objective(s): Proteus mirabilis can cause several diseases have crucial health issues, so current study classified thirty P. mirabilis isolated from UTI into antibiotic resistance level based on phenotypic and genotypic tests.
Material and methods: Genotypically and phenotypically methods were used to identify genes related to antibiotic resistance.
Results: 80% of isolates were resistant to ticarcillin, 81% to mezocillin, and 61% to piperacillin. All isolates were resisting to ticarcillin-clavulante and amoxicillin/clavulanic acid; while 45% of them resisted Ampicillin-Sulbactam. Also, 99% resisted cefepime, 98% resisted ceftazidime and ceftriaxone, and 85% was resistant to cefotaxime. Imipenem was not effective towards 95 % of isolates, while meropenem was effective to 55%. P. mirabilis were resistant to gentamycin, amikacin, and tobramycin in the rate of 75%, 99%, 30%, respectively. Further, 60% resisted tetracycline and 19% resisted doxycycline. Results also showed 90% and 85% were resistant to gatifloxacin and levofloxacin, respectively. Trimethoprime-sulfamethoxazole was non effective among 88%. There was 70% of isolates were resistant to colistin sulfate, and 68% were resistant to tegicycline. MIC test revealed resistancy among 76% of isolates to 32 µg/ml of polymyxin B; while sensitivity were only among P. mirabilis U no. 13, 16, 17, 19, 26, 29, and 30 to 2 µg/ml. Results also demonstrated that only P. mirabilis U26 was classified as MDR, 9/30(30%) were extensive drug resistant and 20/30 (66.6%) were pan drug resistant. Genotypically, 3.3% of isolates had blaIMP-1 and 10% of isolates had bla CMY. Multiplex blaOXA (23, 51, and 58) were detected among (33.3%, 70%, and 26.6%), respectively. Also, there was 80% had aac(3)-I and aac(6)-Ib, and 63.3% had ant(4)IIb- and 96.6% had aph(3). In addition, 66.7% harbored mexX gene and 20% had mexY .Gene rmtA was found in 50% and rmtB was in 27%. Finally, (93%) encoded GyrA. Conclusion: Current study found most P. mirabilis isolates were PDR pathogens among UTI patients in Baghdad hospitals, resulting in health problem issue.
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| Keyword:
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P. mirabilis, Efflux pump, Quinolones, Pan drug resistant, XDR.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.03.505
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| Download:
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