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Screening of anti-inflammatory and antioxidant activities of stink bean (Parkia speciosa Hassk) seed extracts
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| Author:
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NARIN KAKATUM, YUTTANA SUDJAROEN
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| Abstract:
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Stink bean (Parkia speciosa Hassk.) grows naturally in tropical regions like Thailand, Malaysia, Indonesia and Philippines. Seed are beneficial as local food and traditional medicine for various treatments. Aims of this study were to evaluate antioxidant activities of stink bean seed dichloromethane and ethanol extracts by DPPH radical scavenging, lipid peroxidation inhibition and nitric oxide radical scavenging assays; and to evaluate in vitro anti-inflammatory activity. Fresh seeds were removed from pods and prepared in powder form, which was extracted by maceration. Total phenolic content (TPC) measurement stink bean seed extracts were done by Folin-Ciocalteu method. DPPH radical scavenging, lipid peroxidation inhibition and NO radical scavenging activity of extracts were evaluated and compared with butylated hydroxytoluene (BHT), ?-tocopherol and ascorbic acid, respectively. In vitro inflammatory activity of extracts was evaluated and compared with Diclofenac diethylammonium. Dichloromethane extract (89.34±0.17 mg GAE/100 g) from stink bean seed was higher TPC than ethanol extract (62.01±0.03 mg GAE/100 g). Dichloromethane was exhibited DPPH radical scavenging, lipid peroxidation inhibition and in vitro anti-inflammatory activities; and IC50 were 35.43±5.63, 0.77±0.20 and 428.63±87.33 mg/ml, respectively. While, ethanol extract was exhibited DPPH radical scavenging and NO scavenging activities with IC50 were 46.38±7.14 and 58.00±10.76 mg/ml, respectively. It was implied that seed was exhibited anti-inflammatory activity in hydrophobic condition. This preliminary finding may useful data on different of antioxidant activities and anti-inflammatory activity of stink bean seed extract in different conditions.
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| Keyword:
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anti-inflammation, antioxidant activity, Parkia speciosa Hassk., stink bean
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.03.504
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| Download:
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Request For Article
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