|
The Effect of Epigallocatechin Gallate (EGCG) To Ovary Activity and The Expression of LH Receptors in Female Rat (Rattus norvegicus)
|
|
| Author:
|
, DESI WULANSARI D, WIDJIATI, WURLINA, SRI PANTJA MADYAWATI, VISKI FITRI HENDRAWAN, EPY MUHAMMAD
|
| Abstract:
|
Green tea polyphenol, epigallocatechin gallate (EGCG) has been proposing as a cancer chemopreventive, antioxidant and has inhibitor effect in steroidogenesis. The aim of the research was to determine the effect of EGCG on the estrous cycle and expression of LH receptors in female rat (Rattus norvegicus). This research was using 24 female rats, 2-3 months age divided into four groups rat (Rattus norvegicus) were treated with 4.05, 8.1, 12.15 mg EGCG/100 g orally in seven days to study their acute effect on ovarian activity and the expression of luteinizing hormone (LH) receptors. Vagina smear histology has been used as an index of ovarian activity. Daily vagina smears were taken for six days. The expression of LH receptors were examined by immunohistochemistry avidin-biotin complex method. The result showed that EGCG significantly reduced the expression of LH receptors (p < 0.05) and extended follicular phase (0.2-19.4%). The decrease in LH receptors were consistent with an increase in the dose of EGCG. The smallest dose of EGCG (4.05 mg) had given the lowest effect in reduced the expression of LH receptors and extended follicular phase.
|
| Keyword:
|
green tea, Rattus norvegicus, EGCG, ovary activity, expression LH receptor
|
| EOI:
|
-
|
| DOI:
|
https://doi.org/10.31838/ijpr/2021.13.01.028
|
| Download:
|
Request For Article
|
|
|