TLC Bioautography, In Vitro Antiradical and Antioxidant Activities of Leaves Extract of Bauhinia Acuminata Linn. (Caesalpiniaceae)
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Author:
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SUDIPTA CHAKRABORTY, N.N BALA, SUDIPTA DAS
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Abstract:
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Bauhinia acuminata Linn. (Caesalpiniaceae) is a common ornamental plant which has been evaluated for its possible antioxidant properties qualitatively through TLC bioautography and quantitatively through some in vitro testing models including ABTS radical scavenging assay, inhibition of lipid peroxidation etc. The methanolic extract of the leaves revealed to possess chiefly alkaloids and flavonoids and shown significant antioxidant activity by all the methods performed having the best or lowest IC50 value of 91.9 µg/ml in DPPH method. Total phenolic and total flavonoid content were found to be 144.90 ± 4.82 mg gallic acid equivalent/gram dry extract and 114.36 ± 2.80 mg quercetin equivalent/gram dry extract respectively. Ascorbic acid or Trolox was used as standard in different models.
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Keyword:
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Bauhinia acuminata, TLC bioautography, DPPH, ABTS
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EOI:
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-
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DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.630
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