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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
ISSN
0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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In-vitro cytotoxicity screening of symplocos racemosa plant extract by MTT assay

Author: UPLOADED BY-ADMIN, BHUVAN P. RAVAL, MAULIK P. SUTHAR, RAKESH K. PATEL
Abstract: Cancer still remains leading cause of deaths in the world. Traditional plants are valuable source of novel cytotoxic agents and are still playing greater role in health care. Chloroform, butanol and ethyl acetate extracts of Symplocos racemosa bark were prepared and their cytotoxic activity determined using the MTT salt based cytotoxicity assay in 96- micro plate format against three cancers and one normal cell line. Cyclophosphamide was used as positive control. Absorbance was measured after 48 h at 450 nm. Of total 3 extracts evaluated, butanolic and ethyl acetate extracts demonstrated strong cytotoxic potential overall. Assay showed dose depended inhibition of cell growth. Butanolic extract was displayed cytotoxicity against HL 60(Human leukemia cell line, IC50=13058 ng/ml), HeLa(Human cervix cancer cell line, IC50=17155 ng/ml), U-937(Human myeloid lymphoma cancer cell line, IC50=16493 ng/ml), and HEK 293T(Normal human erythrocyte kidney cell line, IC50= 31579 ng/ml) Ethyl acetate extract displayed cytotoxicity against HL60 (IC50=206157 ng/ml), HeLa(IC50=47785 ng/ml) and HEK293T(IC50=267116 ng/ml), and U-937 (IC50=31095 ng/ml). Chloroform extract was inactive against all four cell lies.
Keyword: Symplocos racemosa, MTT, Cytotoxicity, Cell line
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