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The effect of Rhus coriaria L. methanolic extract on cytotoxicity of Cladophora glomerata L. Kützing methanolic extract against human breast carcinoma MCF-7 cell line
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| Author:
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GHAZWAN T.AL-JABER, WAFEEQ N.H.AL-ISMAEEL, ALI A.A.AL-ALI
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| Abstract:
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The present study aimed to evaluate the cytotoxicity of the methanolic extract of Cladophora glomerata macroalga against human breast carcinoma MCF-7 cell line and natural cells (Vero cells) as well as evaluating the combined effect of Rhus coriaria (sumac) methanolic extract. We performed the preliminary phytochemical screening of C. glomerata methanolic extract, then we evaluated the cytotoxicity of C. glomerata methanolic extract against MCF-7 and Vero cell lines using MTT assay with determining the value of Half maximal inhibitory concentration (IC50) of C. glomerata methanolic extract. According to the results C. glomerata methanolic extract contains alkaloids, flavonoids, tannins, saponins, glycosides, triterpenoids, and sterols while it is free of phenols. The C. glomerata methanolic extract has a high cytotoxicity activity against MCF-7 cells compared with Vero cells, the IC50 of the methanolic extract of C. glomerata was 116.7 and 120.4µg/ml against MCF-7 cells and Vero cells respectively. The isobologram analysis results showed that there is antagonism between C. glomerata methanolic extracts and sumac methanolic extracts. The results of the DNA fragmentation analysis showed that there was no degradation of the DNA of MCF-7 cells by using any concentrations of both C. glomerata methanolic extracts and sumac methanolic extracts. We concluded that C. glomerata methanolic extract may be of interest in the cancer treatment field, and we also concluded that the use of sumac methanolic extract for combination did not improve the efficiency of C. glomerata methanol extract.
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| Keyword:
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Cladophora glomerata, Cytotoxicity, MCF-7 cell line, Rhus coriaria
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.008
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| Download:
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