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Effect of Long Exposure Against Quantity and Quality of DNA in Toothpick Samples as an Alternative for Individual Identification
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| Author:
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BETA NOVIA RIZKY, MIEKE SYLVIA MARGARETHA AMIATUN RUTH, AHMAD YUDIANTO
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| Abstract:
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Introduction : Objects exposed to saliva have been shown to be a good and a valuable source of DNA for genotyping in forensic identification. Saliva can be obtained from items used in a person's activities, including cigarette butts, envelopes that are glued together by licking, toothpicks, pillows, and bite marks. The DNA found at crime scenes is often available in very limited, both in quantity and quality. Therefore, PCR amplification technology on DNA is very suitable for analyzing forensic DNA samples because it is sensitivity, fast, also not limited by DNA quality . Loci chosen were FGA and TH01 loci, because the population in Indonesia has several specific alleles that are not shared by other Asian populations.
Method : This was conducted at the Human Genetic Laboratory of Universitas Airlangga, Surabaya, Indonesia, during March 2020. The ethical clearance was issued by the Health Research Ethical Clearance Commission Faculty of Dental Medicine, Universitas Airlangga, under ethical clearance number: 155 / HRECC.FODM / III / 2020. The samples obtained from eight female volunteers who still have complete permanent teeth. Each volunteer repeated the steps until they produced 4 toothpicks. Then, the toothpicks were placed in an envelope and then stored in a room with a stable temperature for 1, 3 and 7 days. Then, DNA extraction process was started, and analysis using PCR. Results: A significant difference of DNA quantity was found quantity on day 1 and day 3, also on the 3rd and 7th day. Meanwhile, there is no significant difference between other variables. The visualization of the electrophoresis results in study was carried out at the FGA and TH01 loci and bands clear.
Discussion : The advantage of toothpicks is that they are easy and comfortable. According to the quantity and quality of DNA in a person's saliva after eating or before eating does not have a difference, so this is possible if someone who has a habit biting a toothpick, then the toothpick can become evidence if it is found at a crime scene. Therefore further research is needed. In addition, this study found that DNA levels on day 0 and 7 did not have a significant difference, so that further research could be carried out with a longer exposure time and in different environmental conditions.
Conclusion : Based on the analysis of the DNA quantity on toothpicks, DNA isolation from toothpick evidence might be an alternative to DNA identification in the forensic field even though it has been left for 7 days.
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| Keyword:
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Forensic, DNA, toothpick, FGA, TH01
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.603
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| Download:
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