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The Cytotoxicity Assays of Hypoxia Mimicking Agent on Rabbit’s (Oryctolagus cuniculus) Gingival Mesenchymal Stem Cells: Experimental In Vitro Study
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| Author:
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ALEXANDER PATERA NUGRAHA, IGO SYAIFUL IHSAN, ARIF NUR MUHAMMAD ANSORI, RADEN JOKO KUNCORONINGRAT SUSILO, HELEN SUSILOWATI, IDA BAGUS NARMADA, DIAH SAVITRI ERNAWATI, FEDIK ABDUL RANTAM
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| Abstract:
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Introduction: Cobalt (II) chloride hexahydrate (CoCl2) is a material that frequently used as hypoxia mimicking agent (HMA) to induce hypoxia condition in the cell culture. CoCl2 may stimulate the adaptive state and maintain the stemness of mesenchymal stem cells (MSCs). Even though CoCl2 commonly administered for cell culture, the biocompabilty of this material should be examined before use on Gingival Mesenchymal Stem Cells (GMSCs). Purpose: the purpose of this study is to examine the biocompability of CoCl2 as HMA on Rabbit’s (Oryctolagus cuniculus GMSCs by using cytotoxicity assay. Materials and Methods: Rabbit’s GMSCs at fourth subculture obtained from cell stock was used in this study. CoCl2 with the concentration as much as 200 ?M, 150 ?M, 100 ?M, 75 ?M, 50 ?M, 25?M was used to be compared in this study. Cytotoxicity test of CoCl2 on GMSCs was conducted by means of 3-(4,5-Dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay. The formazan crystal was observed in the viable cells. The data were analyzed using one-way Analysis of Variance (ANOVA) with p<0.05 considered as significant. Results: The formazan crystals were found in the GMSCs co-cultured with various doses of CoCl2. There was no significant difference in the cell viability percentage of GMSCs between groups treated with CoCl2 with various concentration (p>0.05). Conclusion: CoCl2 as hypoxia mimicking agent are biocompatible to be used on rabbit’s (O. cuniculus) gingival mesenchymal stem cells.
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| Keyword:
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Biocompability, Cobalt (II) chloride hexahydrate, Medicine, Gingival Mesenchymal Stem Cells, Hypoxia Mimicking Agent
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.545
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