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In Vitro Quantitative Assessment of Viability of Echinococcus granulosus Protoscoleces after Treatment with Ruta graveolens L. Methanolic Extract
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| Author:
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, YASEEN TAHA AL QAISI, KHALED M KHLEIFAT, SAWSAN ATALLAH ORAN
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| Abstract:
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The scolicidal agents used to inactivate the protoscoleces through the surgery of hydatid cyst still produce
detrimental side effects. The aim of this study was to find out the in vitro scolicidal effects achievable by Ruta
graveolens methanol extract against the hydatid cysts protoscoleces. The cytotoxic activity of this plant also
was evaluated against fibroblast cells. The yielded extract obtained by methanol extraction of aerial parts of R.
graveolens was about 23.51%%w/w on dry weight basis. The results of this study showed that the methanol
extract of R. graviolens produce phenolic compounds (25.53±0.49), flavonoids (6.66±0.31) and tannins
(8.035±0.049), which greatly reflects their potential as antioxidants. The scolicidal impact of 40 mg/ml of R.
graveolens extract after exposure time of 120 minutes showed very significant efficacy (P<0.05) of
approximately 5-fold of what was obtained at the same period through using the standard drug (ABZ). The
maximum scolicidal impact with 20 mg/mL ABZ as control group was approximately 50% at the longest period
of exposure, 720 min. Nevertheless, all the extract concentrations used resulted in a 100% killing (P <0.05) at
720 min, indicating that the concentration and exposure time were inversely related to the effect on the
viability of the protoscolices. It was found that the use of R. graveolens extract at a concentration of 100 mg/ml
exhibited very low cytotoxicity against the fibroblast with IC50 values of 65.78 mg/ml. Hence, the significant
deadly effects on protoscoleces unravel a novel antihydatic activity of this plant.
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| Keyword:
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Cystic Echinococcosis, hydatid cysts, protoscolices, R. graveolens extract, scolicidal agents.
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.487
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| Download:
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