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Ex vivo, In-vitro and In-silico Neuroprotective Activity of Selected Traditional Medicinal Plants- A Reverse Pharmacological Approach
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| Author:
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Y.MEGHANA , SRIKANTH JEYABALAN
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| Abstract:
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Background: Alzheimer’s disease (AD) is a progressive neurological disorder and is the main cause of dementia, in which brain cells death is observed that causes decrease in memory, language, a problem-solving and other cognitive skills. More than men, women were affected by AD. Alzheimer’s has no cure and the drugs which are available in market have a limited effect. Now-a-days, herbal medicine offers a several options to modify the progress of Alzheimer’s symptoms. These plant products are carefully standardized, and their efficacy, safety was known. Objective: In the present study we have selected 4 traditional medicinal plants i.e., Morinda citrifolia, Withania somnifera, Zingiber officinale, Emblica officinalis to compare in-silico, in-vitro and ex-vivo neuroprotective activity. Materials and methods: The in-silico docking analysis was performed using Schrodinger Maestro v 11.5 for the evaluation of protein-ligand complexes. In vitro studies were performed with acetylcholinesterase obtained from brain of Swiss albino mice. The inhibitory activity was done by ex-vivo studies by comparing the test extracts with the standard drug Neostigmine. Results: By docking analysis it was found that phytoconstituents from Withania somnifera and Emblica officinalis show more binding affinity towards acetylcholinestrase and butylcholinestrase. The bioactivity score was estimated by using Molinspiration cheminformatics. The biological activity was found out by Lipinski rule of 5. From in-vitro and ex-vivo studies it was found that standardised extract of Morinda citrifolia, Withania somnifera and Emblica officinalis show more Neuroprotective activity respectively. Conclusion: The extract of Morinda citrifolia & Emblica officinalis were found to be show more neuroprotective activity in ex vivo studies.
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| Keyword:
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Neurodegenerative disease, Acetylcholinesterase, Medicinal plants, Phytoconstituents.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.551
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| Download:
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