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NEFL gene Expression Analysis in a sample of Iraqi Multiple Sclerosis Patients
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| Author:
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HASSAN LAFTA ATIYAH, ASMAA MOHAMMED SAUD, NAWFAL MADHI SHEAHEED
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| Abstract:
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Multiple sclerosis is a central nervous system chronic disease caused by damage to the myelin covering nerve cells in the brain and spinal cord. Undoubtedly, The multifactorial aetiology of multiple sclerosis, including both genetic and environmental factors. The study investigates the levels of NEFL gene expression to understand the relationship between gene expression and Multiple sclerosis. Collected blood samples were from multiple sclerosis patients in the multiple sclerosis clinic in the Baghdad Teaching Hospital of Medical City Blood samples were taken from fifty volunteers: forty multiple sclerosis patients (12 male and 28 female) and ten healthy controls (3 male and 7 female). RNA extracted and RT-qPCR measured gene expression through two steps: conversion of RNA to cDNA and its amplification. The result shows a significant decrease in expression of NEFL (P=0.05) in the patient's group compared to the control group. While, demographic data between multiple sclerosis subjects and control showed the statistically non-significant result, where p-value for patient and control are (p=0.16). The study also showed environmental factors, including smoking, treatment, and no significant differences (p=0.22, P =0.071 ).
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| Keyword:
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Multiple sclerosis, NEFL gene, Gene expression, RT-qPCR
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.542
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| Download:
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Request For Article
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