|
Development And Evaluation Of Solid-Lipid Nanoparti-cles Loaded With Gemcitabine For Delivery Through Pulmonary Route
|
|
| Author:
|
RACHNA M KUMAR, AFRASIM MOIN, MERRYL DSILVA, SIDDARAMAIAH, D.V. GOWDA
|
| Abstract:
|
Objective: The aim of the research work was to develop and evaluate gemcitabine loaded solid lipid nanoparticles (SLN) for anti-cancer activity through pulmonary route. The SLN acts as drug carriers for both aqueous soluble and poorly soluble medications. Gemcitabine can be used in treatment of different kinds of diseases like breast, ovarian, lung, pancreatic cancer. Method: The solid lipid nanoparticles are prepared by temperature modulated solidification method were used as carriers for gemcitabine. The evaluation parameters include particle size which is measured using zeta sizer and zeta potential of SLNs is noted. Cell viability, rate of hemolysis, rate of permeation study are carried out for the prepared SLN loaded with gemcitabine. The stability characters were determined by storing it at a varying temperature of 4-400 C for a duration of 90 days that are assessed using student t-test. .Result: Formulation R4 was considered optimum with particle size around 20nm and zeta potential of -16.4mV. Cell viability was more for cells treated with free drug than that of drug loaded SLN. Rate of hemolysis expanded with increase in drug concentration. Stability studies showed that there was increase in size with temperature but there was no change in zeta potential. Conclusion: The technique used is simple and reproducible and is considered having the potential for scale-up of solid-lipid nanoparticles formulation containing anti-cancer without the requirement of high energy as well as free from use of organic additives.
|
| Keyword:
|
Gemcitabine; Solid Lipid Nanoparticles; Pulmonary route; Temperature modulated solidification technique.
|
| EOI:
|
-
|
| DOI:
|
https://doi.org/10.31838/ijpr/2021.13.01.562
|
| Download:
|
Request For Article
|
|
|