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Dietary Restriction of Vitamin B12 Induces Hyperhomocysteinemia And Renal Function Impairment in Rat Model
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| Author:
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PATWA AMANI, DEWI IRAWATI SOERIA-SANTOSO, ANI RETNO PRIJANTI, AHMAD AULIA JUSUF, MINARMA SIAGIAN, IRENA UJIANTI, ADITYA KRISHNA MURTHI
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| Abstract:
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Vitamin B12 deficiency is still a global health problem, which mostly caused by lack of intake and malabsorption. Vitamin B12 deficiency is already known associated with hematological and neurological impairment. However, several genetic case studies reported that this condition also affects renal functions. Patients with CblC deficiency, the most common genetic disorder of vitamin B12 metabolism, are reported having various renal manifestations including nephrotic syndrome and thrombotic microangiopathy. This study was conducted to investigate the effect of vitamin B12 diet restriction on kidney functions in rat model. Eighteen male Sprague-Dawley rats were randomly divided into three groups: control group (n=6) was fed standard AIN-93M diet for 12 weeks, 4R group (n=6) was fed vitamin B12-restricted diet for 4 weeks, and 12R group (n=6) was fed vitamin B12-restricted diet for 12 weeks. After 4 and 12 weeks, plasma homocysteine levels were increased in both treatment groups. Kidney function decreased in 4R and 12R groups, marked by increased of plasma creatinine, decreased of creatinine clearance, and increased of urine Kidney Injury Marker-1 (KIM-1). In conclusion, our results support the idea that vitamin B12 diet restriction affects kidney physiological functions.
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| Keyword:
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Vitamin B12, Homocysteine, Kidney Function.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.03.495
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