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Effectiveness of Grapefruit (Citrus Paradisi) And Lime (Citrus Aurantifolia) Against Pathogenic Root Canal Biofilms
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| Author:
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NURANI HAYATI, ARMELIA SARI WIDYARMAN, BOEDI OETOMO ROESLAN
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| Abstract:
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Root canal bacterial biofilms show significant resistance to antimicrobial agents. Citrus as one of the most common consumed fruits contain various phenolic compounds that have antibacterial properties and proven to have low toxicity and unharmful to human tissues. The aim of this study was to examine the effectiveness of grapefruit (Citrus paradisi) and lime (Citrus aurantifolia) in different concentrations in the inhibition and eradication of Porphyromonas endodontalis and Porphyromonas gingivalis biofilm in vitro and their cytotoxicity to human gingival fibroblasts. Pure blended and squeezed juices of grapefruit and lime were diluted in distilled water into concentration 100%, 50% and 25%. The effect of juices toward microbial biofilms were examined using biofilm assay. Chlorhexidine gluconate (2%) and NaOCl (2.5%) was used as a positive control. Data were analyzed using two-way analysis of variance and Tukey’s post-hoc significant difference test with P<0.05 was considered statistically significant. Additionally, MTT cytotoxicity assay was performed to measuring fibroblast viability. The cytotoxicity assay showed that cell viability with each citrus was significantly above the recommended toxicity value for gingival fibroblasts (>90%). The biofilm assays indicated that all grapefruit and lime juice treatments significantly eradicated and inhibited the formation of P. endodontalis and P. gingivalis biofilms in all concentration after exposure for and 15 min and 48 h, respectively (P<0.05). All blended and squeezed-juiced extracts of grapefruit and lime have an antibiofilm effect against P. endodontalis and P. gingivalis. Grapefruit and lime juice may be useful in preventing and eradicating biofilm in root canal.
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| Keyword:
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Biofilm, fibroblast, grapefruit, lime, Porphyromonas endodontalis, Porphyromonas gingivalis.
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.03.494
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| Download:
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