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Determination of Minimum Inhibitory Concentration and Minimum Bactericidal Concentration of Silver Ion Against Staphylococcus Aureus
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| Author:
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RADEN ARIES MUHARRAM, DAVID KAMADJAJA, SENITZA ANISA SALSABILLA, SEPTIANA PUTRINING SUCIADI, HENDRIK SETIA BUDI
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| Abstract:
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Staphylococcus aureus (S. aureus) bacteria are the leading cause of various infections worldwide, including osteomyelitis, infective endocarditis, septicemia and death. The increased resistance of staphylococcus bacteria has resulted in increasing difficulties in the management of this bacterial infection. The use of silver ion as an alternative treatment has been done since ancient times before antibiotics were discovered, and silver ion itself has minimal toxicity and side effects. The aims of this study to determine the MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) of silver ion against the growth of S. aureus. S. aureus was suspended on BHIB media and induced with silver ions with a concentration of 10 ppm, 9 ppm, 8 ppm, 7 ppm, and 6 ppm incubated for 1x24 hours at 37o C to find the MIC value, then streaking each bacterial suspension on the medium Solid MHA to determine the growth of bacterial colonies, then the bacteria were counted using a colony counter and the availability of bacterial was showed green fluoresence microscope on excitation 535 nm, whereas impaired membrane cells present red fluorescence with a maximum excitation of 617 nm of. In the liquid dilution test, the clarity of the solution is shown at a concentration of 7 ppm and in the streaking test, the bacterial colony does not grow at a concentration of 8 ppm, while in the bacterial count it is obtained 12 cfu / mL at a concentration of 7 ppm. Silver ion has potency as an bactericidal on S. aureus. The MIC of silver ion was obtained at a concentration of 7 ppm and the MBC at a concentration of 8 ppm.
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| Keyword:
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staphylococcus aureus, minimum inhibitory concentration, minimum bactericidal concentration, silver ion
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.03.489
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