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Studying Effective Means and Ways to Specifically Prevent Brucellosis in Small Cattle
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| Author:
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MAXIM A. KOSAREV, ALEXEY M. FOMIN, GULNARA M. SAFINA, YANA A. BOGOVA, LILIYA A. TUKHVATULLINA, RAMIL Y. NASIBULLIN , RUFIYA G. KARIMOVA
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| Abstract:
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Brucellosis is a zoonotic disease that causes huge economic losses around the world. The aim of our studies was to develop specific tools and methods for the prevention and elimination of brucellosis in small cattle that would ensure that animals maintain permanent immunity to brucellosis without postvaccinal seropositivity. Sheep were vaccinated with strains B. abortus82, B.melitensis Rev1 and gamma-vaccines of the same strains. Every month, blood was taken from animals for serological tests. One year after immunization, sheep were reimmunized with the live antigen B.abortusR1096. Six months later, they were re-vaccinated. After 6 months, sheep were infected with B. melitensis 16M culture. One month later, the animals were subjected to euthanasia, followed by serological and bacteriological tests to determine the immunological efficacy of the live antigen by background of living and gamma-inactivated vaccines. The double sheep immunization with live antigen at intervals of 6 months (the background of a live vaccine from strain B. abortus 82) fully protected the animals from being infected with the culture of virulent strain B. melitensis 16M. A similar pattern was observed when sheep were reimmunized with the antigen from strain R1096 (the background of a live vaccine from strain B. melitensis Rev1), while the background ? vaccines from strain 82 protected 66.6% of animals against infection, and the background of ? vaccines from strain Rev1 protected only 33.3% of heads. As a result, the live antigen introduced to sheep on the background of live vaccines creates 100% resistance to artificial brucellosis and the number of immune vaccines is half as low in ?-inactivated vaccines. After revaccinations, animals reacted negatively in serological reactions with single brucellosis and ros-bengal antigens and positively in the complement binding reaction with Rantigen. That provides an opportunity to examine animals after revaccination and to remove patients, speeding up the healing of herds from brucellosis.
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| Keyword:
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brucellosis; small cattle; seropositivity; vaccines.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.496
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| Download:
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